广告
为了解不同非洲猪瘟病毒荧光PCR检测试剂盒在检测猪肉及猪肉制品中的特征，采用国内外6种商品化非洲猪瘟病毒实时荧光PCR检测试剂盒，分别对同一样本开展稳定性试验，对相同非洲猪瘟强阳性、中阳性及弱阳性样本开展敏感性试验。
结果显示：6种试剂盒扩增稳定性良好；对强阳性样本，所有商品化试剂盒均能检出；对中阳性及弱阳性样本，特别是弱阳性样本，不同试剂盒的检出情况不尽相同。因此在实际应用中，应根据具体需求选择合适检测试剂盒。本试验为一线工作者选择使用不同商品化非洲猪瘟检测试剂盒提供了一定参考。
Comparison of Different Fluorescent PCR Kits for Detection of ASFV in Pork and Its Products
In order to identify the characteristics of different fluorescent PCR kits for detection of African swine fever virus（ASFV）in pork and its products，stability test was conducted for the same samples by 6 domestic and foreign PCR kits and sensitivity test for the samples with strong，medium and weak positive ASFV. The results showed that all the 6 kits were with good stability；for sensitivity test，the strong positive samples could be detected out by all 6 kits，but medium positive and weak positive samples，especially the latter，the results were quite different depending on different kits. Therefore，an appropriate kit should be selected according to specific need in practice. Some reference was thereby provided for field workers to select various kits.
